HLA-DPB1 Antibody (MHC II) [clone BraFB6] (V2578)

Description

Recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linked alpha (33-35kDa) and beta (27-30kDa) chains. It reportedly reacts with B- & non-T, non-B cell lines but not with T- and myeloid cell lines and leukemias. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders.

Application Notes

Optimal dilution of the HLA-DPB1 antibody should be determined by the researcher.

1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

Immunogen

Non-T, non-B human acute lymphoblastic leukemia REH6 cell line was used as the immunogen for the HLA-DPB1 antibody.

Storage

Store the HLA-DPB1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).