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Home >> Antibodies >> HLA-DPB1 Antibody (MHC II)

HLA-DPB1 Antibody (MHC II) [clone SPM421] (V2579)

  Catalog No Formulation Size Price (USD)  
Image V2579-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V2579-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V2579SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Flow cytometry testing of human Raji cells with HLA-DPB1 antibody (clone SPM421); Red=isotype control, Blue= HLA-DPB1 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name SPM421
Purity Protein G affinity chromatography
UniProt P04440
Localization Cell surface
Applications ELISA (order BSA/sodium azide-free format for coating) :
Flow cytometry : 1-2ug/10^6 cells
Limitations This HLA-DPB1 antibody is available for research use only.
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Description

Recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linked alpha (33-35kDa) and beta (27-30kDa) chains. It reportedly reacts with B- & non-T, non-B cell lines but not with T- and myeloid cell lines and leukemias. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders.

Application Notes

Optimal dilution of the HLA-DPB1 antibody should be determined by the researcher.

Immunogen

Non-T, non-B human acute lymphoblastic leukemia REH6 cell line was used as the immunogen for the HLA-DPB1 antibody.

Storage

Store the HLA-DPB1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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