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Home >> Antibodies >> TXNDC5 Antibody / Thioredoxin domain-containing protein 5

TXNDC5 Antibody / Thioredoxin domain-containing protein 5 (RQ7712)

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  Catalog No Formulation Size Price (USD)  
Image RQ7712 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human colon cancer tissue with TXNDC5 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human colon cancer tissue with TXNDC5 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human prostate adenocarcinoma tissue with TXNDC5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with TXNDC5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectum adenocarcinoma tissue with TXNDC5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human spleen tissue with TXNDC5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human thyroid papillary carcinoma tissue with TXNDC5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human larynx squamous cell carcinoma tissue with TXNDC5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human renal cancer tissue with TXNDC5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse spleen tissue with IHC staining of FFPE rat colon tissue with TXNDC5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat colon tissue with TXNDC5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human 293T, 2) human HeLa, 3) human A549, 4) human HepG2, 5) rat stomach and 6) mouse ovary tissue lysate with TXNDC5 antibody. Predicted molecular weight ~48 kDa.
Flow cytometry testing of human U-87 MG cells with TXNDC5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TXNDC5 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q8NBS9
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This TXNDC5 antibody is available for research use only.
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Description

Thioredoxin domain-containing protein 5 is a protein that in humans is encoded by the TXNDC5 gene. This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal endoplasmic reticulum (ER)-signal sequence, three catalytically active thioredoxin domains and a C-terminal ER-retention sequence. Its expression is induced by hypoxia and its role may be to protect hypoxic cells from apoptosis. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring upstream BLOC1S5 gene.

Application Notes

Optimal dilution of the TXNDC5 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids M109-R403) was used as the immunogen for the TXNDC5 antibody.

Storage

After reconstitution, the TXNDC5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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