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Using mouse Trax as bait in a yeast 2-hybrid screen, Bray et al. (2002) cloned Tsnaxip1 from a mouse testis cDNA library. The deduced protein contains 709 amino acids and has a coiled-coil structure. Mouse Tsnaxip1 shares 74% homology with the predicted human protein. Northern blot analysis of mouse tissues detected a 2.8-kb transcript only in testis. Northern blot analysis of Tsnaxip1 in developing germ cells indicated predominantly postmeiotic expression. Fluorescence-tagged Tsnaxip1 transfected into mouse fibroblasts showed a cytoplasmic distribution and concentrated staining around the nucleus. Predicted to be involved in cell differentiation and spermatogenesis. Predicted to be located in perinuclear region of cytoplasm. Predicted to be active in cytoplasm
Optimal dilution of the TSNAXIP1 antibody should be determined by the researcher.
E. coli-derived recombinant human protein (amino acids M1-E622) was used as the immunogen for the TSNAXIP1 antibody.
After reconstitution, the TSNAXIP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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