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Home >> Antibodies >> TSEN34 Antibody / tRNA-splicing endonuclease subunit Sen34

TSEN34 Antibody / tRNA-splicing endonuclease subunit Sen34 (RQ7701)

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Image RQ7701 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human U-87 MG cells with TSEN34 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human U-87 MG cells with TSEN34 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) rat brain and 2) mouse brain tissue lysate with TSEN34 antibody. Predicted molecular weight ~34 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q9BSV6
Localization Nuclear
Applications Western blot : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This TSEN34 antibody is available for research use only.
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Description

tRNA-splicing endonuclease subunit Sen34 is an enzyme that in humans is encoded by the TSEN34 gene. This gene encodes a catalytic subunit of the tRNA splicing endonuclease, which catalyzes the removal of introns from precursor tRNAs. The endonuclease complex is also associated with a pre-mRNA 3-prime end processing factor. A mutation in this gene results in the neurological disorder pontocerebellar hypoplasia type 2. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Application Notes

Optimal dilution of the TSEN34 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids M1-Q310) was used as the immunogen for the TSEN34 antibody.

Storage

After reconstitution, the TSEN34 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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