TRMT61A Antibody | RQ7670 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	RQ7670	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	429
Formulation

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IHC staining of FFPE human lung adenocarcinoma tissue with TRMT61A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human larynx squamous cell carcinoma tissue with TRMT61A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colon adenocarcinoma tissue with TRMT61A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human breast cancer tissue with TRMT61A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human ovarian serous cancer tissue with TRMT61A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human spleen tissue with TRMT61A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human thyroid cancer tissue with TRMT61A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE mouse brain tissue with TRMT61A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE rat colon tissue with TRMT61A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE rat spleen tissue with TRMT61A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE rat skin tissue with TRMT61A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of FFPE human ovarian cancer tissue with TRMT61A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

Western blot testing of human 1) PC-3, 2) Caco-2, 3) MCF7 and 4) HaCaT cell lysate with TRMT61A antibody. Predicted molecular weight ~31 kDa.

Flow cytometry testing of human HepG2 cells with TRMT61A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TRMT61A antibody.

Flow cytometry testing of human PC-3 cells with TRMT61A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TRMT61A antibody.

Availability	1-3 business days
Species Reactivity	Human, Mouse, Rat
Format	Antigen affinity purified
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit IgG
Purity	Antigen affinity purified
Buffer	Lyophilized from 1X PBS with 2% Trehalose
UniProt	Q96FX7
Localization	Nuclear
Applications	Western blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Flow cytometry : 1-3ug/million cells Immunofluorescence : 5ug/ml Direct ELISA : 0.1-0.5ug/ml
Limitations	This TRMT61A antibody is available for research use only.
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Description

Enables mRNA (adenine-N1-)-methyltransferase activity. Involved in mRNA methylation. Predicted to be located in nucleoplasm. Predicted to be part of tRNA (m1A) methyltransferase complex. Predicted to be active in nucleus.

Application Notes

Optimal dilution of the TRMT61A antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids M1-G289) was used as the immunogen for the TRMT61A antibody.

Storage

After reconstitution, the TRMT61A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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TRMT61A Antibody (RQ7670)

Description

Application Notes

Immunogen

Storage

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Area of Research

Product Type

Protocols

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