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Home >> Antibodies >> TRMT5 Antibody / tRNA methyltransferase 5

TRMT5 Antibody / tRNA methyltransferase 5 (RQ7568)

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  Catalog No Formulation Size Price (USD)  
Image RQ7568 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human U-2 OS cells with TRMT5 antibody (red) and Tubulin Alpha mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human U-2 OS cells with TRMT5 antibody (red) and Tubulin Alpha mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human MCF7 cell lysate with TRMT5 antibody. Predicted molecular weight ~58 kDa, commonly observed at 50-60 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q32P41
Localization Nuclear, cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This TRMT5 antibody is available for research use only.
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Description

tRNAs contain as many as 13 or 14 nucleotides that are modified posttranscriptionally by enzymes that are highly specific for particular nucleotides in the tRNA structure. TRMT5 methylates the N1 position of guanosine-37 (G37) in selected tRNAs using S-adenosyl methionine.

Application Notes

Optimal dilution of the TRMT5 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids R291-E501) was used as the immunogen for the TRMT5 antibody.

Storage

After reconstitution, the TRMT5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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