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Home >> Antibodies >> TRIT1 Antibody / IPT

TRIT1 Antibody / IPT (RQ7537)

  Catalog No Formulation Size Price (USD)  
Image RQ7537 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of human 293T cells with TRIT1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TRIT1 antibody.
Western blot testing of human 1) Jurkat, 2) Raji, 3) K562, 4) RT4, 5) HEL and 6) HeLa cell lysate with TRIT1 antibody. Predicted molecular weight ~53 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q9H3H1
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This TRIT1 antibody is available for research use only.
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Description

tRNA isopentenyltransferase, mitochondrial, also called IPP transferase (IPT) is an enzyme that in humans is encoded by the TRIT1 gene. This gene encodes a protein that that is targeted to the mitochondrion and modifies transfer RNAs (tRNAs) by adding a dimethylallyl group onto the adenine at position 37. This modification is important for maintaining the correct reading frame during protein translation. This gene is considered a tumor suppressor and its expression can decrease cell growth. Alternative splicing results in multiple transcripts variants, most of which are likely non-functional.

Application Notes

Optimal dilution of the TRIT1 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids L16-K415) was used as the immunogen for the TRIT1 antibody.

Storage

After reconstitution, the TRIT1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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