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Home >> Antibodies >> TRIM16 Antibody / Tripartite motif-containing protein 16

TRIM16 Antibody / Tripartite motif-containing protein 16 (RQ7582)

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  Catalog No Formulation Size Price (USD)  
Image RQ7582 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human colorectal adenocarcinoma tissue with TRIM16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human colorectal adenocarcinoma tissue with TRIM16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian serous adenocarcinoma tissue with TRIM16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with TRIM16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with TRIM16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human cervical cancer tissue with TRIM16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate cancer tissue with TRIM16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat colon tissue with TRIM16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) RT4 and 2) HeLa cell lysate with TRIM16 antibody. Predicted molecular weight: 54-63 kDa (multiple isoforms).
Flow cytometry testing of human Daudi cells with TRIM16 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TRIM16 antibody.
Availability 1-3 business days
Species Reactivity Human, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt O95361
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This TRIM16 antibody is available for research use only.
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Description

Tripartite motif-containing protein 16 is a protein that in humans is encoded by the TRIM16 gene. The protein encoded by this gene is a tripartite motif (TRIM) family member that contains two B box domains and a coiled-coiled region that are characteristic of the B box zinc finger protein family. While it lacks a RING domain found in other TRIM proteins, the encoded protein can homodimerize or heterodimerize with other TRIM proteins and has E3 ubiquitin ligase activity. This gene is also a tumor suppressor and is involved in secretory autophagy.

Application Notes

Optimal dilution of the TRIM16 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids M1-Q218) was used as the immunogen for the TRIM16 antibody.

Storage

After reconstitution, the TRIM16 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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