Transcription factor SP1 Antibody | Specificity protein 1

	Catalog No	Formulation	Size	Price (USD)
	RQ7688	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	429
Formulation

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IHC staining of FFPE human ovarian cancer tissue with Transcription factor SP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with Transcription factor SP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human thyroid cancer tissue with Transcription factor SP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lung cancer tissue with Transcription factor SP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colorectal adenocarcinoma tissue with Transcription factor SP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with Transcription factor SP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human glioma tissue with Transcription factor SP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of FFPE human HeLa cells with Transcription factor SP1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of human 1) HeLa, 2) Caco-2 and 3) Jurkat cell lysate with Transcription factor SP1 antibody. Reported molecular weight: 81-95 kDa.

Flow cytometry testing of human A431 cells with Transcription factor SP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Transcription factor SP1 antibody.

Availability	1-3 business days
Species Reactivity	Human
Format	Antigen affinity purified
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG1
Clone Name	3C4C3
Purity	Antigen affinity purified
Buffer	Lyophilized from 1X PBS with 2% Trehalose
UniProt	P08047
Localization	Nuclear, cytoplasmic
Applications	Western blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Immunofluorescence : 5ug/ml Flow cytometry : 1-3ug/million cells
Limitations	This Transcription factor SP1 antibody is available for research use only.
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Description

Transcription factor Sp1, also known as Specificity protein 1 is a protein that in humans is encoded by the SP1 gene. The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene.

Application Notes

Optimal dilution of the Transcription factor SP1 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids Q384-A603) was used as the immunogen for the Transcription factor SP1 antibody.

Storage

After reconstitution, the Transcription factor SP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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Transcription factor SP1 Antibody / Specificity protein 1 [clone 3C4C3] (RQ7688)

Description

Application Notes

Immunogen

Storage

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Area of Research

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Protocols

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