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Home >> Antibodies >> TCF4 Antibody / Transcription factor 4

TCF4 Antibody / Transcription factor 4 (RQ7105)

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  Catalog No Formulation Size Price (USD)  
Image RQ7105 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) human SH-SY5Y, 2) human Daudi, 3) rat testis, 4) rat brain, 5) rat C6, 6) mouse testis, 7) mouse brain and 8) mouse NIH 3T3 cell lysate with TCF4 antibody. Predicted molecular weight ~71 kDa.
Western blot testing of 1) human SH-SY5Y, 2) human Daudi, 3) rat testis, 4) rat brain, 5) rat C6, 6) mouse testis, 7) mouse brain and 8) mouse NIH 3T3 cell lysate with TCF4 antibody. Predicted molecular weight ~71 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P15884
Applications Western blot : 0.5-1ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This TCF4 antibody is available for research use only.
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Description

Transcription factor 4 (TCF-4) also known as immunoglobulin transcription factor 2 (ITF-2) is a protein that in humans is encoded by the TCF4 gene located on chromosome 18q21.2. This gene encodes transcription factor 4, a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed, and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome. In addition, an intronic CTG repeat normally numbering 10-37 repeat units can expand to >50 repeat units and cause Fuchs endothelial corneal dystrophy. Multiple alternatively spliced transcript variants that encode different proteins have been described.

Application Notes

Optimal dilution of the TCF4 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids Q147-V176) was used as the immunogen for the TCF4 antibody.

Storage

After reconstitution, the TCF4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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