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Home >> Antibodies >> STAT3 Antibody

STAT3 Antibody (R32089)

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  Catalog No Formulation Size Price (USD)  
Image R32089 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 419
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IHC staining of FFPE human breast cancer tissue with STAT3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human breast cancer tissue with STAT3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) mouse ANA-1, 2) monkey COS-7, 3) human A549, 4) human K562, 5) rat heart, 6) rat brain, 7) mouse heart and 8) mouse brain tissue lysate with STAT3 antibody. Expected molecular weight: ~92 kDa (alpha) and ~83 kDa (beta).
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat, Monkey
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt P40763
Localization Cytoplasmic, nuclear
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Limitations This STAT3 antibody is available for research use only.
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Description

The transcription factor, signal transducer and activator of transcription-3 (STAT-3) is the most pleiotropic member of the signal transducer and activator of transcription (STAT) family of transcription factors and mediates pivotal responses for the cytokine family. The mouse STAT3 gene contains 24 exons and spans 30 kb. The translation initiation codon is in exon 2, and the stop codon is in exon 24. STAT3 is mapped to 17q21. It contributes to various physiological processes. Hepatic STAT-3 signaling is thus essential for normal glucose homeostasis and may provide new therapeutic targets for diabetes mellitus.

Application Notes

Optimal dilution of the STAT3 antibody should be determined by the researcher.

Immunogen

Amino acids EKQQMLEQHLQDVRKRVQDLEQKMKVVEN of human STAT3 were used as the immunogen for the STAT3 antibody.

Storage

After reconstitution, the STAT3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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