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Home >> Antibodies >> Sphingosine kinase 1 Antibody / SPHK1

Sphingosine kinase 1 Antibody / SPHK1 (RQ7823)

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  Catalog No Formulation Size Price (USD)  
Image RQ7823 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of human HepG2 cells with Sphingosine kinase 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Sphingosine kinase 1 antibody.
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Flow cytometry testing of human HepG2 cells with Sphingosine kinase 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Sphingosine kinase 1 antibody.
Western blot testing of human 1) HeLa, 2) HepG2 and 3) MCF7 cell lysate with Sphingosine kinase 1 antibody. Predicted molecular weight: ~43/51/44 kDa (isoforms 1/2/3).
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q9NYA1
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Sphingosine kinase 1 antibody is available for research use only.
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Description

SPHK1 (Sphingosine Kinase 1), is an enzyme that in humans is encoded by the SPHK1 gene. Melendez et al.(2000) mapped the SPHK1 gene to chromosome 17q25.2 based on sequence identity with ESTs mapped to this region. Kohama et al.(1998) demonstrated that recombinant mouse Sphk1 can specifically phosphorylate D-erythro-sphingosine and that D, L-threo-dihydrosphingosine and N, N-dimethylsphingosine can act as competitive inhibitors of recombinant Sphk1. Pitson et al.(2000) found that recombinant SPHK1 and endogenous SPHK1 purified from placenta had identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties.

Application Notes

Optimal dilution of the Sphingosine kinase 1 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids E55-N357) was used as the immunogen for the Sphingosine kinase 1 antibody.

Storage

After reconstitution, the Sphingosine kinase 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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