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Home >> Antibodies >> SPARC Antibody

SPARC Antibody (F49838)

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  Catalog No Formulation Size Price (USD)  
Image F49838-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F49838-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot analysis of SPARC antibody and Y79 lysate. Predicted molecular weight 35-45 kDa depending on glycosylation level.
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Western blot analysis of SPARC antibody and Y79 lysate. Predicted molecular weight 35-45 kDa depending on glycosylation level.
Western blot analysis of SPARC antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected (2) with the SPARC gene.
IHC analysis of FFPE human skin tissue stained with SPARC antibody
Flow cytometric analysis of WiDr cells using SPARC antibody (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Mouse, Rat, Bovine, Pig, Chicken, Rabbit, Xenopus
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P09486
Applications Western blot : 1:1000
IHC (Paraffin) : 1:10-1:50
Flow Cytometry : 1:10-1:50
Limitations This SPARC antibody is available for research use only.
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Description

Secreted protein acidic and rich in cysteine/osteonectin/BM40, or SPARC, is a matrix-associated protein that elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (ECM).

Application Notes

Titration of the SPARC antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 224-251 from the human protein was used as the immunogen for this SPARC antibody.

Storage

Aliquot the SPARC antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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