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Home >> Antibodies >> Small ribosomal subunit protein uS7 Antibody / RPS5

Small ribosomal subunit protein uS7 Antibody / RPS5 (RQ7761)

  Catalog No Formulation Size Price (USD)  
Image RQ7761 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human placental tissue with Small ribosomal subunit protein uS7 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human lung cancer tissue with Small ribosomal subunit protein uS7 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human HeLa cells with Small ribosomal subunit protein uS7 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human placental tissue with Small ribosomal subunit protein uS7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectum adenocarcinoma tissue with Small ribosomal subunit protein uS7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human testicular seminoma tissue with Small ribosomal subunit protein uS7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung adenocarcinoma tissue with Small ribosomal subunit protein uS7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human 293T, 2) human HeLa, 3) human Jurkat, 4) human HepG2, 5) rat liver, 6) mouse liver and 7) mouse ovary tissue lysate with Small ribosomal subunit protein uS7 antibody. Predicted molecular weight ~23 kDa.
Flow cytometry testing of human U-251 cells with Small ribosomal subunit protein uS7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Small ribosomal subunit protein uS7 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P46782
Localization Cytoplasmic, nuclear
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Small ribosomal subunit protein uS7 antibody is available for research use only.
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Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S7P family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Application Notes

Optimal dilution of the Small ribosomal subunit protein uS7 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids M1-R204) was used as the immunogen for the Small ribosomal subunit protein uS7 antibody.

Storage

After reconstitution, the Small ribosomal subunit protein uS7 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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