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Home >> Antibodies >> SIVA1 Antibody / CD27BP

SIVA1 Antibody / CD27BP (RQ7655)

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  Catalog No Formulation Size Price (USD)  
Image RQ7655 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of human U937 cells with SIVA1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SIVA1 antibody.
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Flow cytometry testing of human U937 cells with SIVA1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SIVA1 antibody.
Flow cytometry testing of human PC-3 cells with SIVA1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SIVA1 antibody.
Western blot testing of human MOLT4 cell lysate with SIVA1 antibody. Predicted molecular weight ~19 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt O15304
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This SIVA1 antibody is available for research use only.
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Description

Apoptosis regulatory protein Siva is a protein that in humans is encoded by the SIVA1 gene. This gene encodes an E3 ubiquitin ligase that regulates cell cycle progression, cell proliferation and apoptosis. The N-terminus of this protein binds to the cytoplasmic tail of the CD27 antigen, a member of the tumor necrosis factor receptor (TNFR) superfamily. In response to UV radiation-induced DNA damage, this protein has been shown to mediate the ubiquitination of proliferating cell nuclear antigen (PCNA), an important step in translesion DNA synthesis.

Application Notes

Optimal dilution of the SIVA1 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids M1-T141) was used as the immunogen for the SIVA1 antibody.

Storage

After reconstitution, the SIVA1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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