Semaphorin-4F Antibody / SEMA4F (RQ7620)

IHC staining of FFPE mouse brain tissue with Semaphorin-4F antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE rat brain tissue with Semaphorin-4F antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human ovarian serous cancer tissue with Semaphorin-4F antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human breast cancer tissue with Semaphorin-4F antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human thyroid cancer tissue with Semaphorin-4F antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human liver cancer tissue with Semaphorin-4F antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lung adenocarcinoma tissue with Semaphorin-4F antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colon adenocarcinoma tissue with Semaphorin-4F antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human larynx squamous cell carcinoma tissue with Semaphorin-4F antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of FFPE human U-2 OS cells with Semaphorin-4F antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of 1) human U-87 MG, 2) rat lung, 3) rat C6 and 4) mouse lung tissue lysate with Semaphorin-4F antibody. Predicted molecular weight ~66 kDa but may be observed at higher molecular weights due to glycosylation.

Flow cytometry testing of human Daudi cells with Semaphorin-4F antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Semaphorin-4F antibody.