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Home >> Antibodies >> Selenocysteine lyase Antibody / SCLY

Selenocysteine lyase Antibody / SCLY (RQ8215)

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Image RQ8215 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human rectum adenocarcinoma tissue with Selenocysteine lyase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human rectum adenocarcinoma tissue with Selenocysteine lyase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human testicular seminoma tissue with Selenocysteine lyase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung adenocarcinoma tissue with Selenocysteine lyase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with Selenocysteine lyase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse kidney tissue with Selenocysteine lyase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat kidney tissue with Selenocysteine lyase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) 293T, 2) HepG2, 3) SH-SY5Y and 4) ThP-1 cell lysate with Selenocysteine lyase antibody. Predicted molecular weight ~48 kDa.
Flow cytometry testing of fixed and permeabilized human SH-SY5Y cells with Selenocysteine lyase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Selenocysteine lyase antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q96I15
Localization Cytoplasm, Nucleus
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Selenocysteine lyase antibody is available for research use only.
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Description

SCLY (Selenocysteine Lyase), is an enzyme that catalyzes the chemical reaction. This enzyme belongs to the family of lyases, specifically the class of carbon-sulfur lyases. The International Radiation Hybrid Mapping Consortium mapped the human SCLY gene to chromosome 2. Mihara et al.(2000) showed that mouse Scly catalyzed the conversion of L-selenocysteine to L-alanine. Scly activity required pyridoxal 5-prime phosphate, was specific to L-selenocysteine, and showed maximum reactivity at pH 9.0.

Application Notes

Optimal dilution of the Selenocysteine lyase antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids E157-A445) was used as the immunogen for the Selenocysteine lyase antibody.

Storage

After reconstitution, the Selenocysteine lyase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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