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- Tel: 858.663.9055
- Email: info@nsjbio.com
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Recognizes a protein of 34kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB). RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation.
Optimal dilution of the RPA32 antibody should be determined by the researcher.
Recombinant human full-length Replication Protein A2 protein was used as the immunogen for the RPA32 antibody.
Aliquot the RPA32 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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