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Home >> Antibodies >> Rho kinase 2 Antibody / ROCK2

Rho kinase 2 Antibody / ROCK2 (RQ7708)

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Image RQ7708 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of human A431 cells with Rho kinase 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Rho kinase 2 antibody.
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Flow cytometry testing of human A431 cells with Rho kinase 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Rho kinase 2 antibody.
Western blot testing of 1) human HeLa, 2) human K562, 3) human U-87 MG, 4) rat PC-12 and 5) mouse lung tissue lysate with Rho kinase 2 antibody. Predicted molecular weight ~161 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt O75116
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Rho kinase 2 antibody is available for research use only.
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Description

Rho-associated kinase (ROCK), including the ROCK-I and ROCK-II isoforms, is a protein kinase involved in signaling from Rho to actin cytoskeleton. Serine/threonine kinase ROCK II/Rho kinase, which is an isozyme of ROCK I, is one of the targets for the small GTPase Rho. ROCK II regulates the formation of actin stress fibers and focal adhesions, cytokinesis, smooth muscle contraction, and the activation of c-fos serum response element. Sequencing analysis has shown that human ROCK II contains 1388 amino acid residues with a calculated molecular mass of approximately 161 kDa. Fluorescence in situ hybridization analysis showed that the human ROCK II gene is located on chromosome 2p24. Thumkeo et al. concluded that ROCK-II is essential in inhibiting blood coagulation and maintaining blood flow in the endothelium-free labyrinth layer and that loss of ROCK-II leads to thrombus formation, placental dysfunction, intrauterine growth retardation, and fetal death.

Application Notes

Optimal dilution of the Rho kinase 2 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids R908-K1386) was used as the immunogen for the Rho kinase 2 antibody.

Storage

After reconstitution, the Rho kinase 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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