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Home >> Antibodies >> Regulatory factor X 3 Antibody / RFX3

Regulatory factor X 3 Antibody / RFX3 (RQ7762)

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  Catalog No Formulation Size Price (USD)  
Image RQ7762 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human U-2 OS cells with Regulatory factor X 3 antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human U-2 OS cells with Regulatory factor X 3 antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE rat brain tissue with Regulatory factor X 3 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
IHC staining of FFPE mouse brain tissue with Regulatory factor X 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with Regulatory factor X 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HEL and 2) SH-SY5Y cell lysate with Regulatory factor X 3 antibody. Predicted molecular weight ~84 kDa.
Flow cytometry testing of human RT4 cells with Regulatory factor X 3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Regulatory factor X 3 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P48380
Localization Nuclear
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Regulatory factor X 3 antibody is available for research use only.
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Description

Transcription factor RFX3 is a protein that in humans is encoded by the RFX3 gene. This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X2, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with other RFX family members. Multiple transcript variants encoding different isoforms have been described for this gene.

Application Notes

Optimal dilution of the Regulatory factor X 3 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids Q283-V749) was used as the immunogen for the Regulatory factor X 3 antibody.

Storage

After reconstitution, the Regulatory factor X 3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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