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Home >> Antibodies >> Recombinant Vinculin Antibody

Recombinant Vinculin Antibody [clone VCL/7091R] (V9615)

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  Catalog No Formulation Size Price (USD)  
Image V9615-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9615-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9615SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human pancreas with recombinant Vinculin antibody (clone VCL/7091R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human pancreas with recombinant Vinculin antibody (clone VCL/7091R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with recombinant Vinculin antibody (clone VCL/7091R) at 2ug/ml. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Vinculin antibody (clone VCL/7091R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name VCL/7091R
Purity Protein A/G affinity
UniProt P18206
Localization Cytoplasm
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant Vinculin antibody is available for research use only.
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Description

Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and -Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.

Application Notes

Optimal dilution of the recombinant Vinculin antibody should be determined by the researcher.

Immunogen

A portion of amino acids 174-322 was used as the immunogen for the recombinant Vinculin antibody.

Storage

Aliquot the recombinant Vinculin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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