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Home >> Antibodies >> Recombinant SMAD4 Antibody

Recombinant SMAD4 Antibody [clone RM277] (R20294)

  Catalog No Formulation Size Price (USD)  
Image R20294-0.1ML Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ul 419
Microvalidated Recrabbitmono
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Western blot of human Jurkat and IMR32 cell lysate using recombinant SMAD4 antibody at 1:500. Predicted molecular weight ~60 kDa.
IHC testing of FFPE human breast cancer tissue with recombinant SMAD4 antibody at 1:2000.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Mouse, Rat
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM277
Purity Protein A purified from animal origin-free supernatant
UniProt Q13485
Gene ID 4089
Applications Immunohistochemistry (FFPE) : 1:1000-1:2000 (1)
Western blot : 1:500-1:1000
Limitations This recombinant SMAD4 antibody is available for research use only.
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Description

This recombinant SMAD4 antibody reacts to human SMAD4 and may also react to mouse or rat Smad4, as predicted by immunogen homology.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant SMAD4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

A peptide corresponding to the C-terminus of human SMAD4 was used as the immunogen for this recombinant SMAD4 antibody.

Storage

Store the recombinant SMAD4 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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