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Home >> Antibodies >> Recombinant Periostin Antibody / POSTN

Recombinant Periostin Antibody / POSTN [clone POSTN/8523R] (V4324)

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  Catalog No Formulation Size Price (USD)  
Image V4324-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4324-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4324SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human lung tissue with recombinant Periostin antibody (clone POSTN/8523R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human lung tissue with recombinant Periostin antibody (clone POSTN/8523R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon tissue with recombinant Periostin antibody (clone POSTN/8523R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Periostin antibody (clone POSTN/8523R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name POSTN/8523R
Purity Protein A/G affinity
UniProt Q15063
Localization Secreted, Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This recombinant Periostin antibody is available for research use only.
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Description

Periostin (PN), also designated osteoblast-specific factor 2 (OSF-2), is a disulfide linked protein originally isolated as a osteoblast-specific factor. Periostin is a secreted protein that binds heparin and functions as a ligand for AlphaVbeta3 and AlphaVbeta5 integrins. In preosteoblasts, Periostin acts as a cell adhesion molecule and plays a role in osteoblast recruitment, spreading and attachment. Periostin is mainly detected in lower gastrointestinal tract, aorta, stomach, placenta, uterus and breast tissues but is up-regulated in epithelial ovarian tumors and overexpressed in breast cancer. Expression of Periostin is increased by bone morphogenetic protein (BMP2) and transforming growth factor beta 1 (TGF beta 1). Periostin contains a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain, which shows homology with the insect protein fasciclin, and a C-terminal domain.

Application Notes

Optimal dilution of the recombinant Periostin antibody should be determined by the researcher.

Immunogen

A recombinant partial protein sequence (within amino acids 193-326) from the human protein was used as the immunogen for the Periostin antibody.

Storage

Aliquot the recombinant Periostin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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