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Home >> Antibodies >> Recombinant Mouse IgM Antibody (Biotin Conjugate)

Recombinant Mouse IgM Antibody (Biotin Conjugate) [clone RM109] (R20170BTN)

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  Catalog No Formulation Size Price (USD)  
Image R20170BTN-50UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 50 ug 419
Microvalidated Recrabbitmono
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ELISA of mouse immunoglobulins shows the recombinant Mouse IgM antibody reacts to mouse IgM; no cross reactivity with IgG1, IgG2a, IgG2b, IgG3, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM109 was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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ELISA of mouse immunoglobulins shows the recombinant Mouse IgM antibody reacts to mouse IgM; no cross reactivity with IgG1, IgG2a, IgG2b, IgG3, IgA, IgE, human IgG, rat IgG, or goat IgG.
ELISA Titration: the plate was coated with different amounts of mouse IgM. A serial dilution of recombinant Mouse IgM antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
Availability 1-3 business days
Species Reactivity Mouse
Format Biotin Conjugate
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM109
Purity Protein A purified from animal origin-free supernatant
UniProt P01872
Gene ID 16019
Applications ELISA : 0.005ug/ml-0.2ug/ml
Limitations This recombinant Mouse IgM antibody is available for research use only.
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Description

This recombinant Mouse IgM antibody reacts to mouse IgM. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG3, IgA, IgE, human IgG, rat IgG, or goat IgG.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Mouse IgM antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Mouse IgM was used as the immunogen for this recombinant Mouse IgM antibody.

Storage

Store the recombinant Mouse IgM antibody at -20oC.

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