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Home >> Antibodies >> Recombinant Mouse IgG2a-Kappa Antibody

Recombinant Mouse IgG2a-Kappa Antibody [clone RM107] (R20165)

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Image R20165-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 419
Microvalidated Recrabbitmono
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Western blot of nonreduced(-) and reduced(+) mouse IgG2a-κ (20 ng/lane), using 0.2ug/ml of recombinant Mouse IgG2a-Kappa antibody. This antibody only reacts to nonreduced Mouse IgG2a-κ.
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Western blot of nonreduced(-) and reduced(+) mouse IgG2a-κ (20 ng/lane), using 0.2ug/ml of recombinant Mouse IgG2a-Kappa antibody. This antibody only reacts to nonreduced Mouse IgG2a-κ.
ELISA of mouse immunoglobulins shows the recombinant Mouse IgG2a-Kappa antibody reacts to the Fab region of mIgG2a-κ; no cross reactivity with IgG2a-lambda, IgG1, IgG3, IgM, IgA, IgE, human/rat/goat IgG.
ELISA titer: the plate was coated with different amounts of mouse IgG2a-κ. A serial dilution of recombinant Mouse IgG2a-Kappa antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
Availability 1-3 business days
Species Reactivity Mouse
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM107
Purity Protein A purified from animal origin-free supernatant
UniProt P01863
Gene ID 380793
Applications ELISA : 0.005ug/ml-0.2ug/ml
Western Blot (non-reduced only) : 0.1-0.5ug/ml
Limitations This recombinant Mouse IgG2a-Kappa antibody is available for research use only.
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Description

This recombinant Mouse IgG2a-Kappa antibody reacts to the Fab region of mouse IgG2a-k. No cross reactivity with IgG2al, IgG1, IgG3, IgM, IgA, IgE, human/rat/goat IgG.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Mouse IgG2a-Kappa antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Mouse IgG was used as the immunogen for this recombinant Mouse IgG2a-Kappa antibody.

Storage

Store the recombinant Mouse IgG2a-Kappa antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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