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Home >> Antibodies >> Recombinant H3K4me2 Antibody

Recombinant H3K4me2 Antibody [clone RM135] (R20201)

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  Catalog No Formulation Size Price (USD)  
Image R20201-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 469
Image
R20201-25UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 25 ug 239
Microvalidated Recrabbitmono
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Western Blot of recombinant histone H3.3 (1) and acid extracts of human HeLa cells (2) using recombinant H3K4me2 antibody at 0.025 ug/ml showed a band of histone H3 dimethylated at Lysine 4 (K4me2) in HeLa cells.
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Western Blot of recombinant histone H3.3 (1) and acid extracts of human HeLa cells (2) using recombinant H3K4me2 antibody at 0.025 ug/ml showed a band of histone H3 dimethylated at Lysine 4 (K4me2) in HeLa cells.
ICC testing of human HeLa cells treated with sodium butyrate using recombinant H3K4me2 antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
The recombinant H3K4me2 antibody specifically reacts to Histone H3 dimethylated at Lysine 4 (K4me2). No cross reactivity with monomethylated (K4me1), trimethylated (K4me3), or other methylations in Histone H3.
Availability 1-3 business days
Species Reactivity All Species
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM135
Purity Protein A purified from animal origin-free supernatant
UniProt P84243
Gene ID 8350
Applications Western blot : 0.02ug/ml-0.2ug/ml
Immunocytochemistry : 1-2ug/ml
ELISA : 0.2-1ug/ml
Limitations This recombinant H3K4me2 antibody is available for research use only.
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Description

This recombinant H3K4me2 antibody reacts to Histone H3 dimethylated at Lysine 4 (K4me2). No cross reactivity with monomethylated Lysine 4 (K4me1) or trimethylated Lysine 4 (K4me3), or other methylation in histone H3.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant H3K4me2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys4) was used as the immunogen for this recombinant H3K4me2 antibody.

Storage

Store the recombinant H3K4me2 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Related Products

   1.   View all Recombinant antibodies

   2.   View all H3K4me2 antibodies

   3.   View all Histone H3 antibodies

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