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Home >> Antibodies >> Recombinant H3K36ac Antibody

Recombinant H3K36ac Antibody [clone RM154] (R20205)

  Catalog No Formulation Size Price (USD)  
Image R20205-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 469
Image
R20205-25UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 25 ug 239
Microvalidated Recrabbitmono
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Western blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate using recombinant H3K36ac antibody at 1 ug/ml showed a band of Histone H3 acetylated at Lysine 36 in treated HeLa cells.
ICC testing of HeLa cells treated with sodium butyrate using the recombinant H3K36ac antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
The recombinant H3K36ac antibody specifically reacts to Histone H3 acetylated at Lysine 36 (K36ac) and is not affected by the phosphorylation of neighboring Ser31. No cross reactivity with acetylated Lysine 4/9/14/18/23/27/56/79/122 in Histone H3.
Availability 1-3 business days
Species Reactivity All Species
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM154
Purity Protein A purified from animal origin-free supernatant
UniProt P84243
Gene ID 8350
Applications Western blot : 0.5-2ug/ml
Immunocytochemistry : 0.5-2ug/ml
ELISA : 0.2-1ug/ml
Limitations This recombinant H3K36ac antibody is available for research use only.
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Description

This recombinant H3K36ac antibody reacts to Histone H3 acetylated at Lysine 36 (K36ac), which is not affected by the phosphorylation of neighboring Ser31. No cross reactivity with other acetylated Lysines in histone H3.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant H3K36ac antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An acetyl-peptide corresponding to the Acetyl-Histone H3 (Lys36) was used as the immunogen for this recombinant H3K36ac antibody.

Storage

Store the recombinant H3K36ac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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