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Home >> Antibodies >> Recombinant Beta Catenin Antibody

Recombinant Beta Catenin Antibody [clone RM276] (R20293)

  Catalog No Formulation Size Price (USD)  
Image R20293-0.1ML Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ul 419
Microvalidated Recrabbitmono
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Western blot of human HeLa and 293 cell lysate using recombinant Beta Catenin antibody at 1:400. Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.
IHC testing of FFPE human breast cancer tissue with recombinant Beta Catenin antibody at 1:1000.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Mouse, Rat
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM276
Purity Protein A purified from animal origin-free supernatant
UniProt P35222
Gene ID 1499
Applications Immunohistochemistry (FFPE) : 1:500-1:1000 (1)
Western blot : 1:400-1:1000
Limitations This recombinant Beta Catenin antibody is available for research use only.
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Description

This recombinant Beta Catenin antibody reacts to human Beta-Catenin (Catenin beta-1) and may also react to mouse or rat Beta-Catenin, as predicted by immunogen homology.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Beta Catenin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

A peptide corresponding to human Beta-Catenin was used as the immunogen for this recombinant Beta Catenin antibody.

Storage

Store the recombinant Beta Catenin antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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