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Home >> Antibodies >> RBM14 Antibody / RNA-binding protein 14

RBM14 Antibody / RNA-binding protein 14 (RQ7824)

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  Catalog No Formulation Size Price (USD)  
Image RQ7824 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human HeLa cells with RBM14 antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human HeLa cells with RBM14 antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) 293T and 3) Daudi cell lysate with RBM14 antibody. Predicted molecular weight ~69 kDa.
Flow cytometry testing of human HeLa cells with RBM14 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RBM14 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q96PK6
Localization Nuclear, cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This RBM14 antibody is available for research use only.
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Description

RNA-binding protein 14 is a protein that in humans is encoded by the RBM14 gene. This gene encodes a ribonucleoprotein that functions as a general nuclear coactivator, and an RNA splicing modulator. This protein contains two RNA recognition motifs (RRM) at the N-terminus, and multiple hexapeptide repeat domain at the C-terminus that interacts with thyroid hormone receptor-binding protein (TRBP), and is required for transcription activation. Alternatively spliced transcript variants encoding different isoforms (with opposing effects on transcription) have been described for this gene.

Application Notes

Optimal dilution of the RBM14 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids M1-M669) was used as the immunogen for the RBM14 antibody.

Storage

After reconstitution, the RBM14 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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