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Ras-related protein Rab-3A is a protein that in humans is encoded by the RAB3A gene. Synapsin I stimulated the Rab3A cycle by increasing GTP binding, GTPase activity, and Rab3A recruitment to the synaptic vesicle membrane. Conversely, Rab3A inhibited Synapsin I binding to actin and Synapsin I-induced synaptic vesicle clustering. It has been found that the protein is necessary for both forms of synaptic plasticity, and it is also involved in calcium exocytosis in neurons. In addition to that, Rab3A has been found to regulate a late step in synaptic vesicle fusion.
The stated application concentrations are suggested starting amounts. Titration of the Rab3A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Amino acids 192-220 (DTADPAVTGAKQGPQLSDQQVPPHQDCAC-human) were used as the immunogen for this Rab3A antibody.
After reconstitution, the Rab3A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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