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Home >> Antibodies >> Prothrombin Antibody

Prothrombin Antibody [clone 5B6G1] (RQ6986)

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  Catalog No Formulation Size Price (USD)  
Image RQ6986 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) human HeLa, 2) human HepG2, 3) human Caco-2, 4) human HL-60, 5) rat liver, 6) rat RH35, 7) mouse liver and 8) mouse HEPA1-6 cell lysate with Prothrombin antibody. Predicted molecular weight ~70 kDa but may be observed at higher molecular weights due to glycosylation.
Western blot testing of 1) human HeLa, 2) human HepG2, 3) human Caco-2, 4) human HL-60, 5) rat liver, 6) rat RH35, 7) mouse liver and 8) mouse HEPA1-6 cell lysate with Prothrombin antibody. Predicted molecular weight ~70 kDa but may be observed at higher molecular weights due to glycosylation.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Clone Name 5B6G1
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P00734
Applications Western blot : 0.5-1 ug/ml
Limitations This Prothrombin antibody is available for research use only.
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Description

F2 (Coagulation Factor II), also known as thrombin, is a serine protease that in humans is encoded by the F2 gene. This gene for human prothrombin (F2) was assigned to chromosome 11p11-q12 by analysis of a panel of somatic cell hybrid DNAs and by in situ hybridization, using both cDNA and genomic probes. The activated thrombin enzyme plays an important role in hemostasis and thrombosis: it converts fibrinogen to fibrin for blood clot formation, stimulates platelet aggregation, and activates coagulation factors V, VIII (F8), and XIII (F13A1). Thrombin also inhibits coagulation by activating protein C.

Application Notes

Optimal dilution of the Prothrombin antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids Y97-R124) was used as the immunogen for the Prothrombin antibody.

Storage

After reconstitution, the Prothrombin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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