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Home >> Antibodies >> PNP Antibody / Inosine phosphorylase / Purine nucleoside phosphorylase

PNP Antibody / Inosine phosphorylase / Purine nucleoside phosphorylase [clone 7G5] (RQ6353)

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  Catalog No Formulation Size Price (USD)  
Image RQ6353 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human A431 cells with PNP antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human A431 cells with PNP antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human lung cancer with PNP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer with PNP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HEK293, 2) PC-3, 3) U-87 MG and 4) A549 cell lysate with PNP antibody. Predicted molecular weight ~32 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b
Clone Name 7G5
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P00491
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence (FFPE) : 5ug/ml
Limitations This PNP antibody is available for research use only.
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Description

The PNP gene encodes purine nucleoside phosphorylase, an enzyme that catalyzes the reversible phosphorolysis of the purine nucleosides and deoxynucleosides inosine, guanosine, deoxyinosine, and deoxyguanosine. It is presented results from gene dosage studies consistent with assignment of the PNP locus to band 14q13. PNP is expressed in most tissues, with markedly greater expression in lymphoid tissues. Genetic deficiencies of PNP result in severely compromised T?lymphocyte function and neurologic dysfunction.

Application Notes

Optimal dilution of the PNP antibody should be determined by the researcher.

Immunogen

Amino acids AMSDAYDRTMRQRALSTWKQMGEQRELQE were used as the immunogen for the PNP antibody.

Storage

After reconstitution, the PNP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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