PFKM Antibody | Fructose 6 Phosphate Kinase

	Catalog No	Formulation	Size	Price (USD)
	F54966-0.4ML	In 1X PBS, pH 7.4, with 0.09% sodium azide	0.4 ml	399
Formulation
	F54966-0.08ML	In 1X PBS, pH 7.4, with 0.09% sodium azide	0.08 ml	185
Formulation

Bulk quote request

›

Western blot testing of human HeLa cell lysate with PFKM antibody. Predicted molecular weight ~85 kDa.

Immunofluorescent staining of human HeLa cells with PFKM antibody (green) and DAPI nuclear stain (blue).

IHC testing of FFPE human skeletal muscle tissue with PFKM antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

IHC testing of FFPE human hepatocellular carcinoma tissue with PFKM antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Availability	1-3 business days
Species Reactivity	Human
Format	Purified
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit Ig
Purity	Purified
UniProt	P08237
Localization	Cytoplasmic
Applications	Immunofluorescence : 1:10-1:50 Immunohistochemistry (FFPE) : 1:50-1:100 Western blot : 1:500-1:1000
Limitations	This PFKM antibody is available for research use only.
	Review this product on BioCompare and get a $20 Amazon gift card

Description

Phosphofructokinase catalyzes the irreversible conversion of fructose 6 phosphate to fructose 1,6 bisphosphate. Mammalian PFK is a complex isozyme consisting of 3 subunits: muscle (M), liver (L), and platelet (P). Only M type PFK isozyme is expressed in mature muscle, while erythrocytes contain both L and M subunits. Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7), also known as Tarui disease.

Application Notes

The stated application concentrations are suggested starting points. Titration of the PFKM antibody may be required due to differences in protocols and secondary/substrate sensitivity.