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Home >> Antibodies >> PFAS Antibody / Phosphoribosylformylglycinamidine synthase

PFAS Antibody / Phosphoribosylformylglycinamidine synthase (RQ6391)

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  Catalog No Formulation Size Price (USD)  
Image RQ6391 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human HeLa cells with PFAS antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human HeLa cells with PFAS antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) placenta, 2) A549, 3) HEK293, 4) HeLa, 5) K562, 6) U937, 7) HepG2 and rat 8) liver, 9) brain, 10) testis and mouse 11) liver, 12) brain and 13) testis tissue lysate with PFAS antibody. Predicted molecular weight ~145 kDa.
Flow cytometry testing of human HepG2 cells with PFAS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PFAS antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt O15067
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunofluorescence (FFPE) : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This PFAS antibody is available for research use only.
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Description

Purines are necessary for many cellular processes, including DNA replication, transcription, and energy metabolism. Ten enzymatic steps are required to synthesize inosine monophosphate (IMP) in the de novo pathway of purine biosynthesis. The enzyme encoded by this gene catalyzes the fourth step of IMP biosynthesis.

Application Notes

Optimal dilution of the PFAS antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids R330-S569) was used as the immunogen for the PFAS antibody.

Storage

After reconstitution, the PFAS antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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