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Home >> Antibodies >> Cd31 Antibody / Pecam-1

Cd31 Antibody / Pecam-1 (RQ6065)

  Catalog No Formulation Size Price (USD)  
Image RQ6065 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of mouse spleen cells with Cd31 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cd31 antibody.
Western blot testing of 1) rat lung and 2) mouse lung lysate with Cd31 antibody. Expected molecular weight: 83-130 kDa depending on level of glycosylation.
Availability 1-3 business days
Species Reactivity Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q08481
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Pecam1 antibody is available for research use only.
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Description

CD31 also known as Platelet endothelial cell adhesion molecule (PECAM-1), is a protein that in human is encoded by the PECAM1 gene. Encoded protein is a member of the immunoglobulin superfamily and this gene is mapped to 17q23.3. CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. It is demonstrated that CD31 expression on human PBSCs may positively affect both neutrophil and platelet engraftment. Meanwhile, CD31 is involved in leukocyte migration and angiogenesis, which are key components of venous thrombus resolution.

Application Notes

Optimal dilution of the Pecam1 antibody should be determined by the researcher.

Immunogen

Recombinant mouse protein (amino acids Q41-E258) was used as the immunogen for the Pecam1 antibody.

Storage

After reconstitution, the Pecam1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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