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Home >> Antibodies >> PDLIM1 Antibody

PDLIM1 Antibody [clone CPTC-PDLIM1-1] (V8195)

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  Catalog No Formulation Size Price (USD)  
Image V8195-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V8195-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V8195SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human breast carcinoma with PDLIM1 antibody (clone CPTC-PDLIM1-1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human breast carcinoma with PDLIM1 antibody (clone CPTC-PDLIM1-1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free PDLIM1 antibody (clone CPTC-PDLIM1-1) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name CPTC-PDLIM1-1
Purity Protein G affinity chromatography
UniProt O00151
Localization Cytoplasmic
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This PDLIM1 antibody is available for research use only.
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Description

CLP36, also known as PDLIM1, CLIM1 or Elfin, is a 329 amino acid cytoplasmic protein that associates with actin stress fibers at the cytoskeleton. Expressed at high levels in skeletal muscle and heart and at lower levels in colon, small intestine, spleen, lung, placenta, kidney, liver, thymus and pancreas, CLP36 functions as a cytoskeletal protein that is thought to act as an adaptor, bringing target proteins to the cytoskeleton. Specifically, CLP36 interacts with Clik1 (-actinin-1, thereby facilitating the association of Clik1 with actin stress fibers. CLP36 contains one PDZ domain and one LIM zinc-binding domain through which it conveys its protein-protein binding capabilities. Human CLP36 shares 88% sequence similarity with its rat counterpart, suggesting a conserved function between species.

Application Notes

Optimal dilution of the PDLIM1 antibody should be determined by the researcher.

Immunogen

A recombinant human full-length PDLIM1 protein was used as the immunogen for this PDLIM1 antibody.

Storage

Store the PDLIM1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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