PDIR Antibody / Protein disulfide isomerase-related protein / PDIA5 (RQ6350)

IHC staining of FFPE human ovarian adenocarcinoma tissue with PDIR antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human rectal cancer tissue with PDIR antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human gallbladder adenocarcinoma tissue with PDIR antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lung cancer tissue with PDIR antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human breast cancer tissue with PDIR antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human adrenocortical adenoma tissue with PDIR antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human placental tissue with PDIR antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of FFPE human Caco-2 cells with PDIR antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of human 1) HeLa, 2) HepG2, 3) Caco-2, 4) ThP-1, 5) MCF7, 6) HL60, 7) A431, 8) T-47D, 9) rat liver, 10) rat lung 11) rat stomach, 12) rat pancreas, 13) mouse liver, 14) mouse lung, 15) mouse stomach and 16) mouse pancreas tissue lysate with PDIR antibody. Predicted molecular weight ~60 kDa.

Flow cytometry testing of human U-2 OS cells with PDIR antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PDIR antibody.