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Home >> Antibodies >> PAX6 Antibody

PAX6 Antibody (F49967)

  Catalog No Formulation Size Price (USD)  
Image F49967-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F49967-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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PAX6 antibody western blot analysis in U251 lysate. Predicted molecular weight ~48kDa.
IHC analysis of FFPE human brain tissue stained with PAX6 antibody
Confocal immunofluorescent analysis of PAX6 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red).
Flow cytometric analysis of HeLa cells using PAX6 antibody (bottom histogram) compared to a negative control (top histogram).
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Mouse, Rat, Bovine, Xenopus
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P26367
Localization Nuclear, cytoplasmic
Applications Western blot : 1:1000
IHC (Paraffin) : 1:10-1:50
Immunofluorescence : 1:10-1:50
Flow Cytometry : 1:10-1:50
Limitations This PAX6 antibody is available for research use only.
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Description

PAX6 is paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the PAX6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 183-210 from the human protein was used as the immunogen for this PAX6 antibody.

Storage

Store at 4oC for up to one month. For long term, aliquot the PAX6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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