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Home >> Antibodies >> Pan-HLA Antibody / HLA-DP/DQ/DR

Pan-HLA Antibody / HLA-DP/DQ/DR [clone rHLA-Pan/8847] (V5135)

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  Catalog No Formulation Size Price (USD)  
Image V5135-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V5135-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V5135SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human ovarian cancer tissue with Pan-HLA antibody (clone rHLA-Pan/8847). Inset: PBS instead of primary, secondary negative control.
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IHC staining of FFPE human ovarian cancer tissue with Pan-HLA antibody (clone rHLA-Pan/8847). Inset: PBS instead of primary, secondary negative control.
SDS-PAGE analysis of purified, BSA-free Pan-HLA antibody (clone rHLA-Pan/8847) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rHLA-Pan/8847
Purity Protein A/G affinity
UniProt P04440 , P01908 , P01909 , P01920 , P01903
Localization Cell Surface
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This Pan-HLA antibody is available for research use only.
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Description

Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an alpha chain (36kDa) and a beta chain (27kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six alpha and ten beta chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response.

Application Notes

Optimal dilution of the Pan-HLA antibody should be determined by the researcher.

Immunogen

Priess cells (a human B cell line) were used as the immunogen for the Pan-HLA antibody.

Storage

Aliquot the Pan-HLA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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