NUMA1 Antibody / Nuclear mitotic apparatus protein 1 (RQ8284)

Immunofluorescent staining of FFPE human U-2 OS cells with NUMA1 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.

IHC staining of FFPE human esophageal squamous carcinoma tissue with NUMA1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human acinar adenocarcinoma of prostate tissue with NUMA1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human breast cancer tissue with NUMA1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lung cancer tissue with NUMA1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Western blot testing of 1) human HeLa, 2) monkey COS-7, 3) human MCF7, 4) human SH-SY5Y, 5) human SiHa, 6) human RT4, 7) rat PC-12 and 8) mouse NIH 3T3 cell lysate with NUMA1 antibody. Predicted molecular weight: 194-238 kDa (multiple isoforms), may be observed at higher molecular weights due to glycosylation.

Flow cytometry testing of fixed and permeabilized human RT4 cells with NUMA1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NUMA1 antibody.