• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Order
Home >> Antibodies >> Nucleolin Antibody

Nucleolin Antibody (RQ5539)

NSJ bio Image Pdf Image
  Catalog No Formulation Size Price (USD)  
Image RQ5539 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
Bulk quote request
Western blot testing of human HepG2 cell lysate with Nucleolin antibody. Predicted molecular weight ~77 kDa, commonly observed at 100~110 kDa.
›
Western blot testing of human HepG2 cell lysate with Nucleolin antibody. Predicted molecular weight ~77 kDa, commonly observed at 100~110 kDa.
Western blot testing of 1) human K562, 2) human HeLa and 3) monkey COS-7 cell lysate with Nucleolin antibody. Predicted molecular weight ~77 kDa, commonly observed at 100~110 kDa.
IHC staining of FFPE human squamous metaplasia of the renal pelvis with Nucleolin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse pancreas with Nucleolin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat pancreas with Nucleolin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of human HL60 cells with Nucleolin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Nucleolin antibody.
Immunofluorescent staining of FFPE human U-2 OS cells with Nucleolin antibody (green) and DAPI nuclear stain (blue).
Immunofluorescent staining of FFPE human A431 cells with Nucleolin antibody (green) and Beta Tubulin antibody (red).
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat, Monkey
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P19338
Localization Nuclear
Applications Western blot : 0.25-0.5ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Flow cytometry : 1-3ug/million cells
Immunofluorescence : 2-4ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This Nucleolin antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

Description

Nucleolin is a protein that in humans is encoded by the NCL gene. It is mapped to 2q37.1. Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.

Application Notes

Optimal dilution of the Nucleolin antibody should be determined by the researcher.

Immunogen

A human recombinant protein (amino acids K219-A629) was used as the immunogen for the Nucleolin antibody.

Storage

After reconstitution, the Nucleolin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Related Products

   1.   View all Nucleolin antibodies

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.