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Home >> Antibodies >> Nucleolin Antibody

Nucleolin Antibody [clone NPC23-2] (V7196)

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  Catalog No Formulation Size Price (USD)  
Image V7196-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7196-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7196SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7196IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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IHC testing of FFPE human tonsil with Nucleolin antibody (clone NPC23-2). FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
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IHC testing of FFPE human tonsil with Nucleolin antibody (clone NPC23-2). FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
Immunofluorescent staining of PFA-fixed human K562 cells with Nucleolin antibody (green, clone NPC23-2) and Phalloidin (red).
Flow cytometry testing of human 293 cells with isotype control and Alexa Fluor 488-labeled Nucleolin antibody (green, clone NPC23-2).
Western blot testing of Nucleolin antibody (clone NPC23-2) and A431 lysate. Predicted molecular weight ~77 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse; Other species not known.
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Clone Name NPC23-2
Purity Protein G affinity chromatography
UniProt P19338
Localization Nucleoli
Applications Immunofluorescence : 1-2ug/ml
Flow cytometry : 1-2ug/million cells in 0.1ml
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This Nucleolin antibody is available for research use only.
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Description

Nucleolin (NCL) is the major nucleolar phosphoprotein of growing eukaryotic cells. It is located mainly in dense fibrillar regions of the nucleolus. NCL is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.

Application Notes

Titering of the Nucleolin antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human protein was used as the immunogen for the Nucleolin antibody.

Storage

Store the Nucleolin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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