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Home >> Antibodies >> Nucleolin Antibody

Nucleolin Antibody (F41619)

  Catalog No Formulation Size Price (USD)  
Image F41619-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F41619-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Nucleolin antibody western blot analysis in HepG2 lysate. Predicted molecular weight is 77 kDa, observed size is 100~110 kDa.
Western blot analysis of Nucleolin antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (2) with the NCL gene.
Nucleolin antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue.
Fluorescent confocal image of HeLa cell stained with Nucleolin antibody at 1:25. NCL immunoreactivity is localized to the nucleus.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Primate
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P19338
Applications Western blot : 1:1000
IHC (Paraffin) : 1:50-1:100
Immunofluorescence : 1:10-1:50
Limitations This Nucleolin antibody is available for research use only.
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Description

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [Wiki]

Application Notes

The stated application concentrations are suggested starting amounts. Due to differences in protocols and secondary/substrate sensitivity, it may be necessary to titrate the Nucleolin antibody for optimal performance.

Immunogen

The amino acid sequence used as immunogen for this Nucleolin antibody is found between 425-455 of the human protein.

Storage

The Nucleolin antibody should be aliquoted and and stored frozen at -20oC. Avoid repeat freeze-thaws.

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