Nucleolar protein 2 Antibody / NOP2 / NSUN1 (RQ8312)

Immunofluorescent staining of FFPE human U-2 OS cells with Nucleolar protein 2 antibody (red), Beta Tubulin mAb (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of human 1) HEL, 2) Caco-2, 3) A549, 4) HeLa, 5) RT4, 6) 293T, 7) SiHa and 8) U-251 cell lysate with Nucleolar protein 2 antibody. Predicted molecular weight: 89 kDa but routinely observed at 100~120 kDa.

Flow cytometry testing of fixed and permeabilized human RT4 cells with Nucleolar protein 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Nucleolar protein 2 antibody.