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Home >> Antibodies >> NUB1 Antibody / NEDD8 ultimate buster 1 / NYREN18

NUB1 Antibody / NEDD8 ultimate buster 1 / NYREN18 (F54770)

  Catalog No Formulation Size Price (USD)  
Image F54770-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54770-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot testing of mouse liver lysate with NUB1 antibody. Expected molecular weight: 70-100 kDa depending on level of ubiquitination.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with NUB1 antibody. Expected molecular weight: 70-100 kDa depending on level of ubiquitination.
IHC testing of FFPE human cancer tissue with NUB1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt Q9Y5A7
Localization Nuclear, cytoplasmic
Applications Western blot : 1:500-1:1000
Immunohistochemistry (FFPE) : 1:25
Limitations This NYREN18 antibody is available for research use only.
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Description

NUB1/NYREN18 is a specific down-regulator of the NEDD8 conjugation system. It recruits NEDD8 and its conjugates to the proteasome for degradation. It also interacts with PSMD4/S5a, a member of the regulatory subunit of the 26S proteasome.

Application Notes

The stated application concentrations are suggested starting points. Titration of the NYREN18 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 87-117 from the human protein was used as the immunogen for the NYREN18 antibody.

Storage

Aliquot the NYREN18 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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