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Home >> Antibodies >> NOX2/gp91phox Antibody / CYBB

NOX2/gp91phox Antibody / CYBB (RQ6611)

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  Catalog No Formulation Size Price (USD)  
Image RQ6611 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of human U937 cells with NOX2/gp91phox antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NOX2/gp91phox antibody.
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Flow cytometry testing of human U937 cells with NOX2/gp91phox antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NOX2/gp91phox antibody.
Western blot testing of human 1) MCF7 and 2) Raji cell lysate with NOX2/gp91phox antibody. Predicted molecular weight ~65 kDa, can be observed at ~85 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P04839
Applications Western blot : 1-2ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This NOX2/gp91phox antibody is available for research use only.
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Description

NOX2 (NADPH OXIDASE 2), also called CYBB (CYTOCHROME b(-245), BETA SUBUNIT), p91-PHOX or GP91-1, is a human gene encoding a glycoprotein. NOX2 is an essential component of phagocytic NADPH-oxidase, a membrane-bound enzyme complex that generates large quantities of microbicidal superoxide and other oxidants upon activation. It is mapped on Xp11.4. NOX2 assembled on DC phagosomes in a gp91-phox subunit-dependent manner, and that reactive oxygen species were produced in a more sustained manner in immature DC phagosomes than in macrophage phagosomes. As a major player in innate immune responses in neutrophils, NOX2 is also involved in adaptive immunity through its activity in DCs. In heart cells, physiologic stretch rapidly activates reduced-form NOX2 to produce reactive oxygen species (ROS) in a process dependent on microtubules (X-ROS signaling).

Application Notes

Optimal dilution of the NOX2/gp91phox antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids F416-H527) was used as the immunogen for the NOX2/gp91phox antibody.

Storage

After reconstitution, the NOX2/gp91phox antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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