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Home >> Antibodies >> NCAPH Antibody / Condensin complex subunit 2

NCAPH Antibody / Condensin complex subunit 2 (RQ8201)

  Catalog No Formulation Size Price (USD)  
Image RQ8201 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human A549 cells with NCAPH antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) 293T and 3) K562 cell lysate with NCAPH antibody. Predicted molecular weight: ~83 kDa, routinely observed at 90-97 kDa.
Flow cytometry testing of fixed and permeabilized human 293T cells with NCAPH antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NCAPH antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q15003
Localization Nuclear, cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This NCAPH antibody is available for research use only.
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Description

Condensin complex subunit 2 also known as chromosome-associated protein H (CAP-H) or non-SMC condensin I complex subunit H (NCAPH) is a protein that in humans is encoded by the NCAPH gene. This gene encodes a member of the barr gene family and a regulatory subunit of the condensin complex. This complex is required for the conversion of interphase chromatin into condensed chromosomes. The protein encoded by this gene is associated with mitotic chromosomes, except during the early phase of chromosome condensation. During interphase, the protein has a distinct punctate nucleolar localization. Alternatively spliced transcript variants encoding different proteins have been described.

Application Notes

Optimal dilution of the NCAPH antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids R91-K721) was used as the immunogen for the NCAPH antibody.

Storage

After reconstitution, the NCAPH antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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