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Home >> Antibodies >> NAT10 Antibody / ALP

NAT10 Antibody / ALP (RQ8333)

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  Catalog No Formulation Size Price (USD)  
Image RQ8333 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human HeLa cells with NAT10 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human HeLa cells with NAT10 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) A549, 2) HeLa and 3) K562 cell lysate with NAT10 antibody. Predicted molecular weight ~116 kDa.
Flow cytometry testing of fixed and permeabilized human HeLa cells with NAT10 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NAT10 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q9H0A0
Localization Nuclear
Applications Western blot : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This NAT10 antibody is available for research use only.
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Description

N-acetyltransferase 10 is an enzyme that in humans is encoded by the NAT10 gene. The protein encoded by this gene is an RNA cytidine acetyltransferase involved in histone acetylation, tRNA acetylation, the biosynthesis of 18S rRNA, and the enhancement of nuclear architecture and chromatin organization.

Application Notes

Optimal dilution of the NAT10 antibody should be determined by the researcher.

Immunogen

An E.coli-derived human recombinant protein (Q22-L740) was used as the immunogen for the NAT10 antibody.

Storage

After reconstitution, the NAT10 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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