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Home >> Antibodies >> NANOG Antibody

NANOG Antibody (F44248)

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  Catalog No Formulation Size Price (USD)  
Image F44248-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F44248-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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IHC analysis of FFPE human testis tissue stained with NANOG antibody
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IHC analysis of FFPE human testis tissue stained with NANOG antibody
Immunofluorescence analysis of NANOG antibody with HeLa cells . Primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.
Flow cytometric analysis of HeLa cells using NANOG antibody (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Bovine, Primate
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Purified
UniProt Q9H9S0
Applications IHC (Paraffin) : 1:10-1:50
Immunofluorescence : 1:10-1:50
Flow Cytometry : 1:10-1:50
Limitations This NANOG antibody is available for research use only.
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Description

NANOG protein is a 305 amino acid protein with a conserved homeodomain motif that is localized to the nuclear component of cells. The homeodomain region facilitates DNA binding. It is a transcription factor in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. NANOG is thought to function in concert with other factors such as POU5F1 (Oct-4) and SOX2 to establish ESC identity. [Wiki]

Application Notes

Titration of the NANOG antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 94-123 from the human protein was used as the immunogen for this NANOG antibody.

Storage

Aliquot the NANOG antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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